P8953

Platelet-Derived Growth Factor from porcine platelets

≥97% (SDS-PAGE and N-terminal analysis), lyophilized powder, suitable for cell culture

• Synonym(e): PDGF
• MDL-Nummer: MFCD00166903
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Produktbezeichnung: Platelet-Derived Growth Factor from porcine platelets, PDGF, lyophilized powder, suitable for cell culture
• Biologische Quelle: Porcine
• Qualitätsniveau: 200
• Assay: ≥97% (SDS-PAGE and N-terminal analysis)
• Form: lyophilized powder
• Wirksamkeit: 39.00 ng/mL EC₅₀
• Qualität: endotoxin tested
• Mol-Gew.: glycoprotein 38 kDa
• Verpackung: pkg of 5 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤1 EU/μg
• Farbe: white
• UniProt-Hinterlegungsnummer: P20034
• Lagertemp.: −20°C
• Angaben zum Gen: pig ... PDGFB(100626465)

Beschreibung

Anwendung

PDGF elicits multifunctional actions with a variety of cells, including mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts.

Biochem./physiol. Wirkung

The principal mitogen found in mammalian serum, released from platelets during clot formation. Elicits mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. Mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. Appears to interact with transforming growth factor-β1 in accelerating wound healing. However, platelet-derived growth factor (PDGF) may also be pathogenic in arteriosclerosis and neoplasia.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid, pH 2.0.

Hinweis zur Analyse

The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)