MSP01

Membran-Gerüstprotein 1D1

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(e): MSP1D1, MSP1T2
• NACRES: NA.26
• UNSPSC Code: 12352200
• Form: buffered aqueous solution
• Assay: ≥90% (SDS-GE)
• Recombinant: expressed in E. coli
• Mol wt: 24,661.6 Da

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥90% (SDS-GE)
• form: buffered aqueous solution
• mol wt: 24,661.6 Da
• solubility: water: soluble
• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

Research area: Cell signaling. Nanodiscs are non-covalent structures that contain a phospholipidbilayer and a membrane scaffold protein (MSP), a genetically engineeredprotein, that mimics the function of Apolipoprotein A-1 (ApoA-1). The firstMSP, MSP1, was engineered with its sequence based on the sequence of A-1, butwithout the globular N-terminal domain of native A-1. The Membrane ScaffoldProtein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in theHelix 1 portion (referred to as “H0.5” in the accompanying figure) of theoriginal MSP1 sequence.It consists of an N-terminal histidine-tagged protein with a tobaccoetch virus (TEV) protease cleavage site that is present between the proteinsequence and histidine-tag.Nano-disctechnology is an approach that allows aqueous soluble membrane proteins in anative-like bilayer environment that enables the membrane proteins to remainactive and stable.

Application

Das Nanodisc-System wird zum Integrieren einer Vielzahl von Proteinen, einschließlich GPCRs, P450s, Bacteriorhodopsin, Gerinnungsfaktoren, Choleratoxin, TAR-Rezeptor und Aromatase, eingesetzt.

Eine umfassende Liste mit Zitaten und Protokollen finden Sie auf der Website von Sligar Lab unter sligarlab.life.uiuc.edu/nanodisc.html

Nanodiscs mit löslichen Lipiddoppelschichten werden in vielen Anwendungen eingesetzt, um Membranproteine in wässrigen Lösungen in einer naturgetreuen Doppelschicht, in der sie ihre Monodispersität und Aktivität aufrechterhalten, löslich zu machen. Die wichtigste Komponente der Nanodiscs ist der umschließende amphipathische helikale Proteingürtel (Membran-Gerüstprotein).

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Biochem/physiol Actions

Zur Herstellung von Nanodiscs mit einem Durchmesser von ~9,7 nm

MSP1D1 can self-assembleinto discoidal nanoparticles known as nano-discs. These nano-discs may be used inthe field of medicine, biotechnology, and membrane protein studies.

Legal Information

Die Nanodisc-Technologie und viele Anwendungen davon sind unter den folgenden Patenten der University of Illinois geschützt.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable