MSP08

Membrane Scaffold Protein 1E3D1

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(e): MSP1E3D1
• NACRES: NA.26
• UNSPSC Code: 12352200
• Form: buffered aqueous solution
• Assay: ≥90% (SDS-GE)
• Recombinant: expressed in E. coli
• Mol wt: 32,599.6 Da

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥90% (SDS-GE)
• form: buffered aqueous solution
• mol wt: 32,599.6 Da
• solubility: water: soluble
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

Research area: Cell Struc

Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 variant of MSP1 differs from MSP1 in the following facets: It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence³ (which is known separately as MSP1D1). It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.

Application

Das Nanodiscsystem wurde eingesetzt, um ein breites Spektrum an Proteinen einzubinden, einschließlich GPCR, P450, Bakteriorhodopsin, Gerinnungsfaktoren, Choleratoxin, TAR-Rezeptor und Aromatase.

Eine ausführliche Liste an Referenzen und Protokollen finden Sie auf der Sligar Lab Website unter sligarlab.life.uiuc.edu/nanodisc.html

Nanodisc lösliche Lipiddoppelschicht-Systeme sind vielseitig anwendbare Mittel, um Membranproteine in wässrigen Lösungen in einer nativ-ähnlichen Doppelschichtumgebung, in der sie monodispergiert und aktiv bleiben, löslich zu machen. Die kritische Komponente von Nanodiscs ist der umschließende amphiphatische, helixförmige Proteingürtel (Membrangerüstprotein).

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Biochem/physiol Actions

Erzeugt Nanodiscs von ~12,9 nm Durchmesser

Legal Information

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

Die Nanodisctechnologie und viele ihrer Anwendungen sind durch die folgenden Patente der University of Illinois geschützt:

• 7.691.414 Membrane scaffold protein
• 7.662.410 Membrane scaffold proteins and embedded membrane proteins
• 7.622.437 Tissue factor compositions and methods
• 7.592.008 Membrane scaffold protein
• 7.575.763 Membrane scaffold proteins and tethered membrane proteins
• 7.083.958 Membrane scaffold protein
• 7.048.949 Membrane scaffold protein

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable