MAK311
Peroxide Assay Kit
sufficient for 250 colorimetric tests
Synonym(e):
Hydrogen Peroxide Test Kit
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Nachweisverfahren
colorimetric
Relevante Krankheit(en)
cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders
Lagertemp.
2-8°C
Verwandte Kategorien
Allgemeine Beschreibung
Peroxide (e.g. hydrogen peroxide H2O2), a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.
Anwendung
Peroxide Assay Kit has been used to measure hydrogen peroxide level in rat tissue homogenates.
Leistungsmerkmale und Vorteile
Compatible with high-throughput handling systems.
Eignung
Suitable for the quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium.
Prinzip
The Peroxide Assay Kit provides a simple and high-throughput adaptable assay for quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. This assay utilizes the chromogenic Fe3+ −xylenol orange reaction, in which a purple complex is formed when Fe2+ is oxidized to Fe3+ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present. The optimized formulation reduces interference by substances in the raw samples. A concentration of 1 μM H2O2 equals 34 ng/mL or 34 ppb. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H2O2 in a 96 well format.
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Lagerklassenschlüssel
8B - Non-combustible corrosive hazardous materials
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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