M5755

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

• Synonym(e): MESA Buffer
• UNSPSC-Code: 12161700
• NACRES: NA.25

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Produktlinie: BioReagent
• Form: powder
• Methode(n): electrophoresis: suitable
• pH-Wert: 6.8-7.2(reconstituted per directions)
• Löslichkeit: H₂O: soluble, faintly yellow
• Fremdaktivität: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Anwendung

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Komponenten

1x MESA buffer contains:

• 40 mM MOPS
• 10 mM sodium acetate
• 1 mM EDTA (pH 8.3)

Rekonstituierung

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)