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D0428

Sigma-Aldrich

1 kb DNA Ladder

for DNA electrophoresis

Synonym(e):

1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

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About This Item

UNSPSC-Code:
41105335
NACRES:
NA.25

Qualitätsniveau

100
200

Form

liquid

Eignung

suitable for electrophoresis (DNA)

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.

Anwendung

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.

Komponenten

Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Sonstige Hinweise

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Rechtliche Hinweise

SYBR is a registered trademark of Life Technologies

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Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000278742
SLCN2917
SLCK0031
SLCJ3543
SLCF6197

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