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Merck

F0504

Sigma-Aldrich

Folatbindendes Protein aus Kuhmilch

lyophilized powder

Synonym(e):

Folate Protein

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.61

Biologische Quelle

bovine milk

Qualitätsniveau

Form

lyophilized powder

Verwendung

1 mg sufficient for ~4,000 assays

Zusammensetzung

Protein, 20-40% modified Warburg-Christian

Methode(n)

ligand binding assay: suitable
surface plasmon resonance (SPR): suitable

Kapazität

1.5-6.0 μg/mg, protein binding capacity (folic acid)(25 °C at pH 7.5.)

Lagertemp.

−20°C

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Anwendung

Folate binding protein can be used in radioligand binding assays of serum folate. The folate receptor has been used in studies as a therapeutic target in a variety of cancers.
Folate binding protein from bovine milk has been used in folate binding studies using surface plasmon resonance and in the uptake studies in kidney proximal tubule cells.
Useful for radioligand binding assays of serum folate. May also bind N-methyltetrahydrofolic acid and other folate analogues.

Biochem./physiol. Wirkung

Folate-binding protein (FBP) is present as a cell receptor and binds to folate in equimolar ratio. It is involved in the transport of folate and improves their bioavailability.

Verpackung

Package size based on protein content

Qualität

Purified over 1,000 fold. May contain a trace of β-mercaptoethanol.

Physikalische Form

Lyophilized powder containing buffer salt as sodium citrate

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Tetrahydrofolates are greatly stabilized by binding to bovine milk folate-binding protein
Jones ML and Nixon PF
The Journal of Nutrition, 132(9), 2690-2694 (2002)
Nikki Parker et al.
Analytical biochemistry, 338(2), 284-293 (2005-03-05)
The folate receptor (FR) is a valuable therapeutic target that is highly expressed on a variety of cancers. The current development of folate-targeted cancer therapies has created the need for quantitating functional FRs in clinical specimens. In this article, we
A radioassay for serum folate: use of a two-phase sequential-incubation, ligand-binding system.
S P Rothenberg et al.
The New England journal of medicine, 286(25), 1335-1339 (1972-06-22)
Tumor imaging using P866, a high-relaxivity gadolinium chelate designed for folate receptor targeting
Corot C, et al.
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, 60(6), 1337-1346 (2008)
The binding of folic acid and 5-methyltetrahydrofolate to folate-binding proteins during gastric passage differs in a dynamic in vitro gastrointestinal model
Verwei M, et al.
The Journal of Nutrition, 134(1), 31-37 (2004)

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