D9905
Denhardt′s Solution
for Northern and Southern blotting, lyophilized powder
Synonym(e):
Denhardt′s Solution For Blotting Technique
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About This Item
UNSPSC-Code:
12161700
NACRES:
NA.25
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Form
lyophilized powder
Methode(n)
Southern blotting: suitable
Fremdaktivität
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
Lagertemp.
2-8°C
Allgemeine Beschreibung
Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Anwendung
Denhardt′s Solution has been used:
- as a component in hybridization buffer for the hybridization of brain sections
- in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
- in the preparation of hybridization mixture
Suitable as a blocking Buffer for Southern and Northern blot membranes.
Biochem./physiol. Wirkung
Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.
Komponenten
Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
Angaben zur Herstellung
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.
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Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Analysenzertifikate (COA)
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