D7290
Deoxyribonucleic acid from herring sperm
single-stranded for hybridization
Synonym(e):
DNA
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
Empfohlene Produkte
Biologische Quelle
(herring sperm)
Qualitätsniveau
Qualität
for molecular biology
Form
solution
Versandbedingung
dry ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Suitable for use as a blocking agent in Southern hybridizations.
Anwendung
Deoxyribonucleic acid from herring sperm is suitable for use as a blocking agent in Southern hybridizations. It was used in preparation of brain sections of mice for BrdU immunohistochemistry. It was also used for hybridization reaction of dot blot membranes.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Angaben zur Herstellung
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Sonstige Hinweise
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
Ähnliches Produkt
Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Simon A Joosse et al.
BMC cancer, 7, 43-43 (2007-03-09)
Array Comparative Genomic Hybridization (aCGH) is a rapidly evolving technology that still lacks complete standardization. Yet, it is of great importance to obtain robust and reproducible data to enable meaningful multiple hybridization comparisons. Special difficulties arise when aCGH is performed
Hanbin Ma et al.
Scientific reports, 3, 2730-2730 (2013-09-26)
Herein, we describe a novel integrated biosensor for performing dielectric spectroscopy to analyze biological samples. We analyzed biomolecule samples with different concentrations and demonstrated that the solution's impedance is highly correlated with the concentration, indicating that it may be possible
Minna M Poranen et al.
Journal of virology, 80(16), 8081-8088 (2006-07-29)
Virus-induced changes in cellular gene expression and host physiology have been studied extensively. Still, there are only a few analyses covering the entire viral replication cycle and whole-host gene pool expression at the resolution of a single gene. Here we
Marloes Prins et al.
PloS one, 8(12), e83835-e83835 (2014-01-01)
Multiple sclerosis (MS) has been mainly attributed to white matter (WM) pathology. However, recent evidence indicated the presence of grey matter (GM) lesions. One of the principal mediators of inflammatory processes is interleukin-1β (IL-1β), which is known to play a
Chromosome 17 and 21 aneuploidy in buccal cells is increased with ageing and in Alzheimer's disease.
Philip Thomas et al.
Mutagenesis, 23(1), 57-65 (2007-12-01)
Alzheimer's disease (AD) is a premature ageing syndrome characterized by cognitive impairment arising from neuropathological changes occurring within specific areas of the brain. We report a 1.5-fold increase in trisomy 21 (P < 0.001) and a 1.2-fold increase in trisomy
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.