CRISPR12
CRISPR-Lenti Non-Targeting Control Plasmid
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About This Item
UNSPSC-Code:
41106609
NACRES:
NA.51
Empfohlene Produkte
Form
liquid
Qualitätsniveau
Verpackung
vial of 50 μL
Konzentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
Anwendung(en)
CRISPR
Versandbedingung
dry ice
Lagertemp.
−20°C
Allgemeine Beschreibung
The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA (non-targeting), with both Puromycin and GFP co-expressed with Cas9.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), originally found in bacteria and archaea, is employed by organisms to protect them from invading viruses and plasmids.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), originally found in bacteria and archaea, is employed by organisms to protect them from invading viruses and plasmids.
Anwendung
Functional Genomics/Screening/Target Validation
To see more application data, protocols, vector maps visit sigma.com/crisprs.
- Non-targeting control, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines.
To see more application data, protocols, vector maps visit sigma.com/crisprs.
Leistungsmerkmale und Vorteile
Serves as a non-targeting or negative experimental control (DNA format) for the CRISPR editing workflow using lenti CRISPRs. This allows you to examine the effect of transfection in your system with the Sigma lenti CRISPR/Cas9 plasmid.
Physikalische Form
The Sigma CRISPR Lenti Non-Targeting Control Vector is provided as 1 μg of plasmid DNA in Tris-EDTA (TE).
Rechtliche Hinweise
Lagerklassenschlüssel
10 - Combustible liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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