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C0897

Sigma-Aldrich

Citratlyase aus Klebsiella pneumoniae

lyophilized powder, ≥0.20 unit/mg solid

Synonym(e):

Citrate oxaloacetate lyase

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About This Item

CAS-Nummer:
9012-83-3
EC-Nummer:
4.1.3.6 (BRENDA, IUBMB)
EG-Nummer:
232-740-7
MDL-Nummer:
MFCD00130807
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

lyophilized powder

Qualitätsniveau

200

Spezifische Aktivität

≥0.20 unit/mg solid

Mol-Gew.

>5 kDa

Lagertemp.

2-8°C

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Anwendung

Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).

Biochem./physiol. Wirkung

Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).

Einheitendefinition

U setzt 1.0 μmol Citrat um zu Oxalacetat pro Minute bei pH 7.6 und 25°C.

Physikalische Form

Lyophilisiertes Pulver, enthält Rinderserumalbumin, Saccharose, MgSO4 und EDTA

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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M Hupperich et al.
European journal of biochemistry, 192(1), 161-166 (1990-08-28)
The primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from Klebsiella pneumoniae (ATCC 13,882) was determined with protein chemical methods. The polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 Da.
Djamel Drider et al.
Genetics and molecular research : GMR, 3(2), 273-281 (2004-07-22)
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert
M Martín et al.
FEMS microbiology letters, 174(2), 231-238 (1999-05-26)
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF
Citric acid mediates the iron absorption from low molecular weight human milk fractions
Palika R, et al.
Journal of Agricultural and Food Chemistry, 61(46), 11151-11157 (2013)
M Suzuki et al.
Archives of microbiology, 175(1), 75-78 (2001-03-29)
Hydrogenobacter thermophilus strain TK-6 was observed to grow anaerobically on nitrate as an electron acceptor when molecular hydrogen was used as an energy source. Nitrite was detected as the product of a respiratory reaction. 15NO, 15N2O, and 15N2 were detected
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