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Sigma-Aldrich

Anti-c-Myc

clone 9E10, purified from hybridoma cell culture

Synonym(e):

Anti-c-Myc, Monoclonal Anti-c-Myc−Biotin antibody produced in mouse

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.56

Biologische Quelle

mouse

Qualitätsniveau

200

Konjugat

biotin conjugate

Antikörperform

purified from hybridoma cell culture

Antikörper-Produkttyp

primary antibodies

Klon

9E10, monoclonal

Form

buffered aqueous solution

Speziesreaktivität

human

Konzentration

0.8 mg/mL

Methode(n)

western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein

UniProt-Hinterlegungsnummer

P01106

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... MYC(4609)

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Allgemeine Beschreibung

The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.

Spezifität

Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).

Immunogen

Synthetisches Peptid des menschlichen p62c-Myc-Proteins.

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
The antibody is suitable for immunoblotting.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000214425
0000205585
119M4770V
22190617
108M4870V

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Yan Wang et al.
PloS one, 7(12), e52404-e52404 (2012-12-29)
The neural cell adhesion molecule L1 plays important roles in neuronal migration and survival, neuritogenesis and synaptogenesis. L1 has also been found in tumors of different origins, with levels of L1 expression correlating positively with the metastatic potential of tumors.
Illari Salvatori et al.
Journal of neurochemistry, 146(5), 585-597 (2018-05-21)
TAR DNA-binding protein 43 (TDP-43) is an RNA-binding protein and a major component of protein aggregates found in amyotrophic lateral sclerosis and several other neurodegenerative diseases. TDP-43 exists as a full-length protein and as two shorter forms of 25 and
Steven R Bates et al.
Applied physics letters, 95(7), 73705-73705 (2009-09-25)
We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association
Ilja Demuth et al.
Oncogene, 23(53), 8611-8618 (2004-10-07)
DNA interstrand crosslinks (ICLs) are critical lesions for the mammalian cell since they affect both DNA strands and block transcription and replication. The repair of ICLs in the mammalian cell involves components of different repair pathways such as nucleotide-excision repair
Himari Ogino et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(12), 3181-3191 (2017-02-19)
The secreted glycoprotein Reelin regulates embryonic brain development and adult brain functions. It has been suggested that reduced Reelin activity contributes to the pathogenesis of several neuropsychiatric and neurodegenerative disorders, such as schizophrenia and Alzheimer's disease; however, noninvasive methods that
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