B5525

Bromphenolblau Natriumsalz

• Synonym(e): 3′,3′′,5′,5′′-Tetrabrom-phenolsulfophthalein Natriumsalz
• Empirische Formel (Hill-System): C₁₉H₉Br₄NaO₅S
• CAS-Nummer: 34725-61-6
• Molekulargewicht: 691.94
• Beilstein: 6995701
• EG-Nummer: 252-170-2
• MDL-Nummer: MFCD00013793
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 57653941
• NACRES: NA.52

Eigenschaften

• Form: powder
• Qualitätsniveau: 200
• Methode(n): electrophoresis: suitable
• Löslichkeit: water: soluble
• Eignung: suitable for nucleic acid electrophoresis
• Lagertemp.: room temp
• SMILES String: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
• InChIKey: TWKDJMRHSLWRFI-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Anwendung

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.

Ein Tracking-Farbstoff für Nuklein- oder Proteinelektrophorese in Agarose- oder Polyacrylamidgels.

Rekonstituierung

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)