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Merck

A3672

Sigma-Aldrich

Azurin aus Pseudomonas aeruginosa

from Pseudomonas aeruginosa, lyophilized powder

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About This Item

CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.61

Biologische Quelle

Pseudomonas aeruginosa

Qualitätsniveau

Form

lyophilized powder

Zusammensetzung

Protein, ≥65% Lowry

Konzentration

≥65.0% (Lowry)

Methode(n)

toxicology assay: suitable

Löslichkeit

water: soluble 1—1.1 mg/mL, clear, blue (light blue to blue)

UniProt-Hinterlegungsnummer

Lagertemp.

−20°C

Angaben zum Gen

Pseudomonas aeruginosa ... AZU(878046)

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Allgemeine Beschreibung

Research area: Apoptosis. Azurinis a periplasmic protein and is a homotetramer.

Anwendung

Azurin has been used:
  • in the cytotoxicity and cell viability studies in human osteosarcoma cell line
  • for the functionalization of silicon nitride cantilevers for interaction studies
  • for coating gold surface and insulating functionalized oxide surfaces of silicon oxide and mica

Biochem./physiol. Wirkung

Azurin acts as an electron donor for nitrite reductase in bacterial denitrification process. It exhibits anticancer activity as it hampers various independent signaling pathways associated with cancer progression. It binds to tumor suppressor protein p53 and induces cancer cell apoptosis or stalls cancer cell growth. Azurin disrupts angiogenesis by reducing the activity of VEGFR-2tyrosine kinase thereby inhibiting tumor growth. It has been observed to show cytotoxicity in human breast cancer cells and human melanoma cells.
Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition.

Physikalische Form

Lyophilized powder containing ammonium acetate buffer salts.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Caterina Bernini et al.
Journal of the American Chemical Society, 135(12), 4822-4833 (2013-03-06)
Many biological electron-transfer reactions involve short-lived tryptophan radicals as key reactive intermediates. While these species are difficult to investigate, the recent photogeneration of a long-lived neutral tryptophan radical in two Pseudomonas aeruginosa azurin mutants (Az48W and ReAz108W) made it possible
Koyu Fujita et al.
Journal of inorganic biochemistry, 115, 163-173 (2012-08-23)
Pseudoazurin (PAz), a well-characterized blue copper electron-transfer protein, is shown herein to be capable of mediating electron transfer to the nitrous oxide reductase (N(2)OR) from Achromobacter cycloclastes (Ac). Spectroscopic measurements demonstrate that reduced PAz is efficiently re-oxidized by a catalytic
Meng Gao et al.
Protein science : a publication of the Protein Society, 26(12), 2334-2341 (2017-09-30)
Azurin secreted by Pseudomonas aeruginosa is an anticancer bacteriocin, which preferentially enters human cancer cells and induces apoptosis or growth inhibition. It turns out that azurin is a multi-target anticancer agent interfering in the p53 signaling pathway and the non-receptor
Comparison of the self-chemisorption of azurin on gold and on functionalized oxide surfaces
Schnyder BK, et al.
Surface and Interface Analysis : SIA, 34(1), 40-44 (2002)
Nadav Amdursky et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(2), 507-512 (2012-12-26)
Measuring solid-state electron transport (ETp) across proteins allows studying electron transfer (ET) mechanism(s), while minimizing solvation effects on the process. ETp is, however, sensitive to any static (conformational) or dynamic (vibrational) changes in the protein. Our macroscopic measurements allow extending

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