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Merck

A3415

Sigma-Aldrich

Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Anti Sheep Antibody, Anti Sheep Antibody - Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey, Anti Sheep Hrp

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

donkey

Qualitätsniveau

Konjugat

peroxidase conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

sheep

Methode(n)

direct ELISA: 1:10,000

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Peroxidase antibody reacts with all sheep immunoglobulins.

Immunogen

Purified sheep IgG

Anwendung

Anti-Sheep IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
rElisas and CHEkiT Elisas were performed on serum from Sarda milking ewes using HRP-conjugated donkey anti-sheep IgG at1:1000. The antibody was incubated for 30 minutes at 37 degrees.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative

Angaben zur Herstellung

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramme

Exclamation markEnvironment

Signalwort

Warning

Gefahreneinstufungen

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Marcus G Davey et al.
PloS one, 12(1), e0171132-e0171132 (2017-02-01)
A major limitation to adeno-associated virus (AAV) gene therapy is the generation of host immune responses to viral vector antigens and the transgene product. The ability to induce immune tolerance to foreign protein has the potential to overcome this host
Increased contractility of diabetic rabbit corpora smooth muscle in response to endothelin is mediated via Rho-kinase β
Chang, S., et al.
The Journal of Urology, 15, 53-62 (2003)
Max Werth et al.
Hepatology (Baltimore, Md.), 44(4), 967-975 (2006-09-29)
In mammalian liver, high glutamine synthetase (GS) expression is restricted to hepatocytes surrounding the terminal venules. The most important enhancer of the GS gene is located approximately 2520 base pairs (bp) upstream from the transcriptional start point. The nature of
María J Torres et al.
Frontiers in microbiology, 8, 1621-1621 (2017-09-16)
The powerful greenhouse gas, nitrous oxide (N
S Chang et al.
International journal of impotence research, 15(1), 53-62 (2003-02-28)
Corpus cavernosum smooth muscle (CCSM) from rabbits made diabetic for 6 months as a result of alloxan injection exhibited increased sensitivity (3vs 9 nM EC(50)) and generated 20-50% greater force to endothelin-1 (ET-1) compared to CCSM from normal rabbits. In

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