88417

Thiazolylblau-formazan

MTT reduction end product, powder

• Synonym(e): 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, MTT-Formazan
• Empirische Formel (Hill-System): C₁₈H₁₇N₅S
• CAS-Nummer: 57360-69-7
• Molekulargewicht: 335.43
• MDL-Nummer: MFCD00080731
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 329769650
• NACRES: NA.83

Eigenschaften

• product name: Thiazolylblau-formazan, MTT reduction end product
• Qualitätsniveau: 100
• Form: powder
• Löslichkeit: chloroform: 10 mg/mL, clear, dark violet
• SMILES String: Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
• InChI: 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
• InChIKey: GIFKVQOPAJDJLO-SZVLODPRSA-N

Beschreibung

Anwendung

MTT Formazan has been used in cell viability assay.

Biochem./physiol. Wirkung

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Sonstige Hinweise

Reviews: Tetrazolium salts and formazans

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)