77618

Phenol–Chloroform–Isoamylalkohol-Mischung

BioUltra, for molecular biology, 49.5:49.5:1

• Synonym(e): phenol:Chloroform:Isoamyl Alcohol
• CAS-Nummer: 136112-00-0
• MDL-Nummer: MFCD00133763
• UNSPSC-Code: 12352401
• PubChem Substanz-ID: 329767817
• NACRES: NA.07

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 100
• Produktlinie: BioUltra
• Form: liquid
• Verunreinigungen: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• pH-Wert: 7.7-8.3(H₂O-phase, after extraction with H₂O, 1:1)
• Dichte: 1.29 g/mL at 20 °C
• Absorption: cut-off at 360 nm
• Lagertemp.: 2-8°C
• SMILES String: ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
• InChI: 1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
• InChIKey: ZYWFEOZQIUMEGL-UHFFFAOYSA-N

Beschreibung

Anwendung

• Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022).


• Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018).


• The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015).


• The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013).

Physikalische Form

Mischung aus Phenol; Chloroform; Isoamylalkohol im Verhältnis von 49.5:49.5:1 (V/V/V); gesättigt mit 100 mM TRIS pH 8.0; enthält ~0.1% 8-Hydroxy-chinolin

Sonstige Hinweise

Large- and small-scale phenol extractions

Sicherheitshinweise

• Piktogramme: GHS06,GHS08,GHS05,GHS09
• Signalwort: Danger
• H-Sätze: H301,H312 + H332,H314,H336,H341,H351,H361d,H372,H373,H411
• P-Sätze: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3
• Zielorgane: Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin
• Lagerklassenschlüssel: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): 177.8 °F - closed cup
• Flammpunkt (°C): 81 °C - closed cup
• Persönliche Schutzausrüstung: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter