I9392

3-Methylbutanol

for molecular biology, BioReagent, ≥98.5%

• Synonym(e): Isoamylalkohol, Isopentylalkohol
• Lineare Formel: (CH₃)₂CHCH₂CH₂OH
• CAS-Nummer: 123-51-3
• Molekulargewicht: 88.15
• Beilstein: 1718835
• EG-Nummer: 204-633-5
• MDL-Nummer: MFCD00002934
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 24896157
• NACRES: NA.52

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Produktlinie: BioReagent
• Assay: ≥98.5%
• Form: liquid
• Methode(n): RNA extraction: suitable
• pH-Wert: 5.6 (20 °C, 25 g/L)
• Dichte: 0.809 g/mL at 20 °C (lit.)
• Eignung: suitable for nucleic acid purification
• Lagertemp.: room temp
• SMILES String: CC(C)CCO
• InChI: 1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3
• InChIKey: PHTQWCKDNZKARW-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

3-methylbutanol (isoamyl alcohol) is a clear, colorless alcohol commonly used as an organic solvent . It can also be used in phenol-chloroform nucleic acid extraction and purification protocols, where it inhibits RNase activity and reduces foaming. Isoamyl alcohol can also be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation.

Anwendung

3-Methylbutanol (isoamyl alcohol) in combination with phenol and chloroform is used in extraction of RNA. It was used in the synthesis of oligo-RNAs using photocaged adenosine molecules.

3-Methylbutanol has been used as a component of phenol/chloroform/ isoamyl alcohol solution for DNA extraction from blood and from absorbing substrata based forensic samples and biofilms.

Sicherheitshinweise

• Piktogramme: GHS02,GHS05,GHS07
• Signalwort: Danger
• H-Sätze: H226,H315,H318,H332,H335
• P-Sätze: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 1
• Flammpunkt (°F): 110.3 °F - closed cup
• Flammpunkt (°C): 43.5 °C - closed cup
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter