55689

Alkoholdehydrogenase equine

recombinant, expressed in E. coli, ≥0.5 U/mg

• Synonym(e): ADH
• CAS-Nummer: 9031-72-5
• EC-Nummer: 1.1.1.1 (BRENDA, IUBMB)
• EG-Nummer: 232-870-4
• MDL-Nummer: MFCD00081305
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: equine
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Beschreibung: Isozyme E sequence
• Form: lyophilized powder
• Spezifische Aktivität: ≥0.5 U/mg
• Farbe: white; light yellow
• pH-Wert: 7
• Löslichkeit: water: 5 mg/mL
• Anwendung(en): life science and biopharma
• Lagertemp.: −20°C
• Angaben zum Gen: equine ... ADH1(111772995)

Beschreibung

Allgemeine Beschreibung

Research Area: Neuroscience
Alcohol dehydrogenase is a zinc metalloprotein that forms five classes of isoenzymes through the dimerization of eight different subunits.

Anwendung

Alcohol Dehydrogenase equine has been used in in vitro alcohol dehydrogenase (Adh) assay.

Biochem./physiol. Wirkung

Alcohol dehydrogenase catalyzes the oxidative conversion of alcohol into aldehyde. It has a homodimeric structure with a co-enzyme binding domain at the C-terminal and an N-terminal catalytic domain. The active site is located at the interdomain cleft. Binding of NAD+ in the active site causes conformational changes which create the binding site for the alcohol substrate.

Horse liver alcohol dehydrogenase (HL-ADH) is an enzyme with broad specificity, capable of catalyzing the reversible oxidation of a wide variety of primary and secondary alcohols to form their corresponding aldehydes and ketones. Moreover, alcohol dehydrogenase can oxidize ethanol while simultaneously reducing nicotinamide adenine dinucleotide (NAD+) to NADH. Previous studies have demonstrated that ADH and ALDH variants can influence alcohol dependence. Additionally, the ADH genotype has been linked to lacunar infarction and neuropsychiatric diseases.

Einheitendefinition

1 U corresponds to the amount of enzyme which reduces 1 μmol benzaldehyde per minute at pH 7.0 and 30 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable