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Merck

5006

Sigma-Aldrich

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol

Synonym(e):

Collagen Type I, PureCol Collagen

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

EG-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.75

Produktbezeichnung

PureCol, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

Qualitätsniveau

Sterilität

sterile-filtered

Assay

99.9%

Form

lyophilized

Verpackung

pkg of 15 mg

Methode(n)

cell culture | mammalian: suitable

Bindungsspezifität

Peptide Source: Elastin

Peptide Source: Fibrinogen

Versandbedingung

wet ice

Lagertemp.

−20°C

Allgemeine Beschreibung

PureCol collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol, lyophilized form is not recommended for the formation of a solid gel. PureCol collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Anwendung

PureCol has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Angaben zur Herstellung

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Rechtliche Hinweise

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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