T3163

Tetramethylrhodamin-isothiocyanat Isomer R

powder

• Synonym(e): 6-TRITC, Tetramethylrhodamin-6-isothiocyanat
• Empirische Formel (Hill-System): C₂₅H₂₁N₃O₃S
• CAS-Nummer: 80724-20-5
• Molekulargewicht: 443.52
• MDL-Nummer: MFCD00063517
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24900112
• NACRES: NA.47

Eigenschaften

• Beschreibung: labeling efficiency with bovine albumin >= 50%
• Qualitätsniveau: 200
• Form: powder
• Zusammensetzung: Dye content, ≥80%
• Löslichkeit: 1 M NH4OH: 10 mg/mL (Faint Red to Very Dark Red to Very Dark Purple solution)
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-8°C
• SMILES String: CN(C)c1ccc2c(OC3=C\C(C=CC3=C2c4cc(ccc4C([O-])=O)N=C=S)=[N+](/C)C)c1
• InChI: 1S/C25H21N3O3S/c1-27(2)16-6-9-19-22(12-16)31-23-13-17(28(3)4)7-10-20(23)24(19)21-11-15(26-14-32)5-8-18(21)25(29)30/h5-13H,1-4H3
• InChIKey: OBYNJKLOYWCXEP-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

Anwendung

• TRITC is used to label a wide variety of biomolecules, including immunoglobulins, lectins, nucleic acids, polynucleotides, and polysaccharides for affinity, immuno-, and in situ hybridization, fluorescent probes, and flow cytometry.
• Lectins are TRITC-Iabeled reagents for the affinity staining of sections and cell monolayers to distinguish the sialomucins of salivary glands.
• TRITC-antibodies are used to identify pathogenic amoebae.
• TRITC-oligonucleotides are used in the in-situ hybridization staining of soil microorganisms.
• TRITC-Iabeled reagents have also been used as fluorescent probes of live cells.
• Flow cytometry of TRlTC-Iabeled slime mold cells has been used to study their aggregation behavior.

Sicherheitshinweise

• H-Sätze: H412
• P-Sätze: P273
• Gefahreneinstufungen: Aquatic Chronic 3
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable