F3651

Fluoreszein-5(6)-isothiocyanat

≥90% purity (HPLC), powder

• Synonym(e): FITC
• Empirische Formel (Hill-System): C₂₁H₁₁NO₅S
• CAS-Nummer: 27072-45-3
• Molekulargewicht: 389.38
• Beilstein: 1407295
• EG-Nummer: 248-207-7
• MDL-Nummer: MFCD00005063
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24894855
• NACRES: NA.47

Eigenschaften

• Produktbezeichnung: Fluoreszein-5(6)-isothiocyanat, ≥90% (HPLC)
• Qualitätsniveau: 200
• Assay: ≥90% (HPLC)
• Form: powder
• Methode(n): titration: suitable
• Farbe: yellow to orange
• Löslichkeit: acetone: 1 mg/mL, clear, light yellow to yellow
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-8°C
• SMILES String: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChIKey: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]

Anwendung

Für die Fluoreszenzmarkierung von Proteinen (z.B. Immunofluoreszenz); H Protein-Labeling für die Gelchromatographie.

Fluorescein 5(6)-isothiocyanate has been used:

• for fluorescence labeling of bacteria[3]
• as a standard for calibration of fluorometer[4]
• for the labeling of exosomes[5]

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3