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Merck

RDD029

Sigma-Aldrich

Guanidinthiocyanat

for molecular biology, free-flowing, Redi-Dri, ≥99.0%

Synonym(e):

Guanidin-rhodanid, Guanidiniumthiocyanat

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About This Item

Lineare Formel:
NH2C(=NH)NH2 · HSCN
CAS-Nummer:
Molekulargewicht:
118.16
Beilstein:
3563461
MDL-Nummer:
UNSPSC-Code:
12352107
NACRES:
NA.28

Produktlinie

Redi-Dri

Qualitätsniveau

Assay

≥99.0%

Qualität

for molecular biology
free-flowing

mp (Schmelzpunkt)

120-122 °C (lit.)

Fremdaktivität

DNAse, none detected
RNAse, none detected

SMILES String

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChIKey

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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Allgemeine Beschreibung

Guanadine thiocyanate (GTC) is a chaotropic agent and strong denaturant that is used in research for protein degredation in vitro. This is useful for DNA or RNA isolation and purification. GTC is a key reagent for inactivation of endogenous DNases and RNase for molecular biology techniques used to isolate nucliec acids from tissues, cells, or viral genomes.

Anwendung

Chaotropisches Reagens und starker Denaturant; löst Zellen auf
Guanidine thiocyanate is commonly used for research:

  • to denature proteins in cell lysis buffer
  • to isolate and purify RNA from cells and tissues
  • to extract DNA from cellular and viral genomes

Biochem./physiol. Wirkung

Guanadine thiocyanate is strong protein denaturant commonly combinded with phenol to lyse tissues, cells, and proteins during RNA isolation assay.

Leistungsmerkmale und Vorteile

  • Redi-Dri makes working with hygroscopic chemicals easier
  • Controls moisture and powder remains free-flowing
  • Safer to handle and weigh out for routine use in lab
  • Eliminates clumps and maintains product moisture and quality attributes

Sonstige Hinweise

For additional information on our range of Biochemicals, please complete this form.

Rechtliche Hinweise

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

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Preisangaben

Piktogramme

CorrosionExclamation mark

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Zusätzliche Gefahrenhinweise

Lagerklassenschlüssel

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

Martin Roszkowski et al.
Frontiers in cell and developmental biology, 9, 648274-648274 (2021-05-11)
The extraction of high-quality ribonucleic acid (RNA) from tissues and cells is a key step in many biological assays. Guanidinium thiocyanate-phenol-chloroform (AGPC) is a widely used and efficient method to obtain pure RNA from most tissues and cells. However, it
Peter L Roberts et al.
Biologicals : journal of the International Association of Biological Standardization, 35(4), 343-347 (2007-05-23)
Virus inactivation by a number of protein denaturants commonly used in gel affinity chromatography for protein elution and gel recycling has been investigated. The enveloped viruses Sindbis, herpes simplex-1 and vaccinia, and the non-enveloped virus polio-1 were effectively inactivated by
Serazhutdin A Abdullaev et al.
Radiation and environmental biophysics, 54(3), 297-304 (2015-05-04)
Investigation of cell-free DNA (cf-DNA) in body fluids, as a potential biomarker for assessing the effect of ionizing radiation on the organism, is of considerable interest. We investigated changes in the contents of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear

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