G6639

Guanidinthiocyanat

≥97% (titration)

• Synonym(e): Guanidin-rhodanid, Guanidiniumthiocyanat
• Lineare Formel: NH₂C(=NH)NH₂ · HSCN
• CAS-Nummer: 593-84-0
• Molekulargewicht: 118.16
• Beilstein: 3563461
• EG-Nummer: 209-812-1
• MDL-Nummer: MFCD00013027
• UNSPSC-Code: 12352209
• PubChem Substanz-ID: 24895264
• NACRES: NA.55

Eigenschaften

• Biologische Quelle: synthetic
• Qualitätsniveau: 200
• Assay: ≥97% (titration)
• Form: powder or crystals
• Methode(n): RNA extraction: suitable
• Farbe: white to off-white
• mp (Schmelzpunkt): 120-122 °C (lit.)
• Löslichkeit: water: 4 M, clear, colorless
• Anionenspuren: chloride (Cl^-): ≤2.5%
• SMILES String: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChIKey: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Beschreibung

Anwendung

Guanidine thiocyanate has been used:

• as a denaturing agent to study its effects on surface activity of globular protein
• for the extraction of total RNA from primary hepatocytes
• as a component of the incubating buffer for extraction of DNA from human bone
• as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
• as a component of lysis solution for extraction of RNA from rat pancreatic tissues

Chaotropisches Reagens und starker Denaturant; löst Zellen auf

Biochem./physiol. Wirkung

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.

Sicherheitshinweise

• Piktogramme: GHS05,GHS07
• Signalwort: Danger
• H-Sätze: H302 + H312 + H332,H314,H412
• P-Sätze: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• Zusätzliche Gefahrenhinweise: EUH032
• Lagerklassenschlüssel: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves