PPB010

Tris EDTA Buffer, pH 8.0

pHast Pack^™, powder, 10X concentrate

• Synonym(e): 10X TE buffer
• UNSPSC-Code: 12161700
• NACRES: NA.25

Eigenschaften

• Produktlinie: pHast Pack^™
• Form: powder
• Lagerbedingungen: (Tightly closed. Dry)
• Verunreinigungen: ≤5 ppm Heavy Metals (lead)
• Farbe: white
• pH-Wert: 7.9—8.1
• Löslichkeit: soluble, clear, colorless
• Kationenspuren: Fe: <10 ppm
• Fremdaktivität: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

Anwendung

TE buffer is intended for use in:

• Nucleic acid gel electrophoresis
• storage and dilution of DNA and RNA
• DNA automated sequencing
• Immunohistochemistry

Leistungsmerkmale und Vorteile

• Fast and easy Ready-to-use buffer
• Minimizes effort - no need to weigh and adjust pH
• Saves time and eliminates exposure to hazardous chemicals
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Verpackung

Foil pouch

Rekonstituierung

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Lagerung und Haltbarkeit

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Sonstige Hinweise

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Rechtliche Hinweise

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Warning
• H-Sätze: H373
• P-Sätze: P260 - P314 - P501
• Gefahreneinstufungen: STOT RE 2 Inhalation
• Zielorgane: Respiratory Tract
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable