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Merck

D8189

Sigma-Aldrich

Bis(1,3-dibutylbarbitursäure)-Trimethinoxonol

≥95% purity (HPLC), powder or solid

Synonym(e):

DiBAC4(3)

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About This Item

Empirische Formel (Hill-System):
C27H40N4O6
CAS-Nummer:
Molekulargewicht:
516.63
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.47

product name

Bis(1,3-dibutylbarbitursäure)-Trimethinoxonol, ≥95% (HPLC)

Qualitätsniveau

Assay

≥95% (HPLC)

Form

powder or solid

Methode(n)

titration: suitable

Farbe

orange to dark red

Löslichkeit

DMF: soluble
DMSO: soluble

ε (Extinktionskoeffizient)

≥90000 at 490-496 nm in methanol

Fluoreszenz

λex 513-519 nm

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

2-8°C

SMILES String

CCCCN(C(N(CCCC)C1=O)=O)C(C1=C/C=C/C2C(N(CCCC)C(N(CCCC)C2=O)=O)=O)=O

InChI

1S/C27H40N4O6/c1-5-9-16-28-22(32)20(23(33)29(26(28)36)17-10-6-2)14-13-15-21-24(34)30(18-11-7-3)27(37)31(25(21)35)19-12-8-4/h13-15,20H,5-12,16-19H2,1-4H3/b14-13+

InChIKey

ATJCYSYHWGQAET-BUHFOSPRSA-N

Anwendung

Bis(1,3-dibutylbarbituric acid) trimethine oxonol has been used for monitoring changes in the plasma membrane potential. It also has applications in high throughput screening.
Anwendung im Hochdurchsatz-Screening.

Biochem./physiol. Wirkung

Bis(1,3-dibutylbarbituric acid) trimethine oxonolis a translational membrane potential dye that is redistributed in the cell as membrane potential changes.
Translational membrane potential dye that is redistributed in the cell as membrane potential changes.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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T Bräuner et al.
Biochimica et biophysica acta, 771(2), 208-216 (1984-04-11)
The usefulness of a new voltage-sensitive fluorescent dye, the membrane permeant negatively charged oxonol dye diBA-C4-(3)-, was evaluated by measuring the membrane potentials of BICR/M1R-k and L cells with glass microelectrodes and simultaneously recording the fluorescence of the stained cells.
B Lee et al.
Journal of applied microbiology, 127(3), 701-712 (2019-06-20)
Metal nanoparticles are promising materials for the management of infectious diseases as known to have various antimicrobial activities in pathogenic micro-organisms. Among them, gold nanoparticles (AuNPs) are used in a wide range of fields such as photodynamic therapy, molecular diagnostics
Johannes Kassmannhuber et al.
Bioengineered, 8(5), 488-500 (2017-01-26)
In a concept study the ability to induce heterogeneous ice formation by Bacterial Ghosts (BGs) from Escherichia coli carrying ice nucleation protein InaZ from Pseudomonas syringae in their outer membrane was investigated by a droplet-freezing assay of ultra-pure water. As
Easa Nagamalleswari et al.
Nucleic acids research, 45(14), 8423-8434 (2017-09-01)
Programmed cell death (PCD) under certain conditions is one of the features of bacterial altruism. Given the bacterial diversity and varied life style, different PCD mechanisms must be operational that remain largely unexplored. We describe restriction endonuclease (REase) mediated cell
Autocrine regulation of glioma cell proliferation via pHe-sensitive K(+) channels.
Honasoge A, et al.
American Journal of Physiology. Cell Physiology, 306, C493-C505 (2014)

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