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Merck

21879

Sigma-Aldrich

5(6)-Carboxyfluoreszeindiacetat

BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Synonym(e):

5(6)-Carboxy-di-O-acetylfluorescein

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About This Item

Empirische Formel (Hill-System):
C25H16O9
CAS-Nummer:
Molekulargewicht:
460.39
MDL-Nummer:
UNSPSC-Code:
12352108
PubChem Substanz-ID:
NACRES:
NA.32

Produktlinie

BioReagent

Qualitätsniveau

Assay

≥90.0% (HPLC)

Form

powder

Löslichkeit

DMSO: soluble

Fluoreszenz

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

Eignung

suitable for fluorescence

Lagertemp.

2-8°C

SMILES String

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1.CC(=O)Oc6ccc7c(Oc8cc(OC(C)=O)ccc8C79OC(=O)c%10cccc(C(O)=O)c9%10)c6

InChI

1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)

InChIKey

SWRGCNMDGRMQGB-UHFFFAOYSA-N

Allgemeine Beschreibung

5(6)-Carboxyfluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.

Anwendung

5(6)-Carboxyfluorescein diacetate (CFDA) is a dye which is been extensively utilized to label cultured neurons with the essential requirements, in a specific manner.

5(6)- Carboxyfluorescein diacetate (CFDA) is a viability probe that assesses enzymatic activity, which is necessary to activate its fluorescence, and cell-membrane integrity, which is necessary for the retention of their fluorescent product within the cell.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Zhuo Chen et al.
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R E Petroski et al.
Journal of neuroscience methods, 52(1), 23-32 (1994-04-01)
A method for selectively labeling cultured neurons using the vital dye, 5(6)-carboxyfluorescein diacetate (CFDA), is described. This non-fluorescent membrane-permeant dye is cleaved by cytosolic esterases into the fluorescent anion, 5(6)-carboxyfluorescein (CF). Both astrocytes and neurons exhibit brilliant fluorochromasia within minutes
Rodrigo Anibal Mateos-Nava et al.
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