69899

Monochlorbiman

suitable for fluorescence, ≥70.0% (HPCE)

• Synonym(e): Chlorbiman
• Empirische Formel (Hill-System): C₁₀H₁₁ClN₂O₂
• CAS-Nummer: 76421-73-3
• Molekulargewicht: 226.66
• Beilstein: 4440901
• MDL-Nummer: MFCD00077379
• UNSPSC-Code: 12352108
• PubChem Substanz-ID: 329762099
• NACRES: NA.32

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥70.0% (HPCE)
• Form: powder
• mp (Schmelzpunkt): 135-136 °C (lit.)
• Löslichkeit: DMF: soluble; DMSO: soluble; acetonitrile: soluble; methanol: soluble
• Fluoreszenz: λ_ex 380 nm; λ_em 461 nm in methanol; λ_ex 390 nm; λ_em 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)
• Eignung: suitable for fluorescence
• SMILES String: CC1=C(C)C(=O)N2N1C(CCl)=C(C)C2=O
• InChI: 1S/C10H11ClN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
• InChIKey: SUIPVTCEECPFIB-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Monochlorobimane is a glutathione (GSH) fluorescent cell-permeable probe. When incubated with the test cell culture, it readily enters the cells and forms a fluorescent complex. The Monochlorobimane-GSH reaction is catalyzed by glutathione-S-transferase, which is detected fluorometrically.

Monochlorobimane, also known as mBCl, is a non-fluorescent compound that forms a fluorescent complex upon reaction. The fluorescence is detected at 394/490nm.

Anwendung

Monochlorobimane is used as a fluorescent agent in fluorometric glutathione assays. It is used to detect the principal intracellular low-molecular-weight thiols, which play a pivotal role in the defense mechanism.

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H315,H319,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves