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Merck

11426338910

Roche

Anti-Fluorescein-AP, Fab fragments

from sheep

Synonym(e):

antibody

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203

Biologische Quelle

sheep

Qualitätsniveau

Konjugat

alkaline phosphatase conjugate

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

solution

Verpackung

pkg of 150 U (200 μl)

Hersteller/Markenname

Roche

Isotyp

IgG

Lagertemp.

2-8°C

Allgemeine Beschreibung

Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)

Spezifität

The polyclonal antibody reacts with free and bound fluorescein.

Anwendung

Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
  • Dot blot
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Southern blot
  • Western blot
  • Electrochemical biosensing technique for the detection of 16S rRNA

Biochem./physiol. Wirkung

Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).

Leistungsmerkmale und Vorteile

Contents
Solution, stabilized

Angaben zur Herstellung

  • Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
  • ELISA: 150 to 300mU/ml
  • Immunohistocytochemistry: 250 to 500mU/ml
  • In situ hybridization: 1.5 to 7.5U/ml
  • Southern blot: 150mU/ml
  • Western blot: 250 to 500 mU/ml

Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

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Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Skin Sens. 1

WGK

WGK 1

Flammpunkt (°F)

No data available

Flammpunkt (°C)

No data available


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