PC213L
Anti-GABA Rabbit pAb
lyophilized, Calbiochem®
Synonym(e):
Anti-Gamma Amino-Butyric Acid
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About This Item
UNSPSC-Code:
12352203
NACRES:
NA.43
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
serum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
lyophilized
Enthält nicht
preservative
Speziesreaktivität
rat
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
Isotyp
IgG
Versandbedingung
ambient
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
rat ... Gabra1(29705)
Allgemeine Beschreibung
Anti-GABA, rabbit polyclonal, recognizes GABA in rat thalamus and cerebellum. It is validated for use in immunohistochemistry on frozen sections.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes GABA.
Recognizes GABA in rat thalamus and cerebellum.
Immunogen
GABA
Rat
Anwendung
Frozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
Warnhinweis
Toxicity: Standard Handling (A)
Physikalische Form
Undiluted serum.
Rekonstituierung
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Hinweis zur Analyse
Positive Control
Rat thalamus or cerebellum
Rat thalamus or cerebellum
Sonstige Hinweise
Antibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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