MAB312
Anti-A2B5 Antibody, clone A2B5-105
clone A2B5-105, Chemicon®, from mouse
Synonym(e):
Neuron Cell Surface Antigen
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
A2B5-105, monoclonal
Speziesreaktivität (Voraussage durch Homologie)
mammals
Hersteller/Markenname
Chemicon®
Methode(n)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
Isotyp
IgM
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Spezifität
Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).
Immunogen
Embryonic chicken retinal cells
Anwendung
Research Category
Neurowissenschaft
Neurowissenschaft
Research Sub Category
Neuronen- & Gliamarker
Neuronen- & Gliamarker
Detect A2B5 using this Anti-A2B5 Antibody, clone A2B5-105 validated for use in FC, IC, IF, IH.
Indirect immunofluorescence (1:100 - 1:500) in the detection of neurons in tissue culture.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.
Physikalische Form
Format: Purified
Liquid in 0.02M PB, 0.25M NaCl, pH 7.6 containing 0.1% sodium azide.
Lagerung und Haltbarkeit
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Hinweis zur Analyse
Control
Type II astrocytes, human neural progenitors
Type II astrocytes, human neural progenitors
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Noah M Walton et al.
Development (Cambridge, England), 133(18), 3671-3681 (2006-08-18)
The isolation and expansion of human neural cell types has become increasingly relevant in restorative neurobiology. Although embryonic and fetal tissue are frequently envisaged as providing sufficiently primordial cells for such applications, the developmental plasticity of endogenous adult neural cells
Sebastián L Vega et al.
Experimental cell research, 351(1), 11-23 (2016-12-31)
Stem and progenitor cells that exhibit significant regenerative potential and critical roles in cancer initiation and progression remain difficult to characterize. Cell fates are determined by reciprocal signaling between the cell microenvironment and the nucleus; hence parameters derived from nuclear
Hala Gabr et al.
Cell transplantation, 24(9), 1813-1827 (2014-09-10)
Spinal cord injury (SCI) results in demyelination of surviving axons, loss of oligodendrocytes, and impairment of motor and sensory functions. We have developed a clinical strategy of cell therapy for SCI through the use of autologous bone marrow cells for
Brittney A Beyer et al.
Nature chemical biology, 14(1), 22-28 (2017-11-14)
Endogenous metabolites play essential roles in the regulation of cellular identity and activity. Here we have investigated the process of oligodendrocyte precursor cell (OPC) differentiation, a process that becomes limiting during progressive stages of demyelinating diseases, including multiple sclerosis, using
Jeremy A Murphy et al.
Investigative ophthalmology & visual science, 52(10), 7771-7777 (2011-08-30)
To characterize the influence of endothelin-1 (ET-1) on optic nerve head astrocyte (ONHA) proliferation and Ca²⁺ signaling in ONHAs lacking functional endothelin B (ETB) receptors. ONHAs were isolated from adult wild type (WT) and transgenic spotting lethal (TSL) rats, lacking
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