MAB2029
Anti-CSPG4 Antibody
CHEMICON®, mouse monoclonal, 9.2.27
Synonym(e):
Anti-CSPG4A, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Produktbezeichnung
Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27, clone 9.2.27, Chemicon®, from mouse
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
9.2.27, monoclonal
Speziesreaktivität
human
Hersteller/Markenname
Chemicon®
Methode(n)
flow cytometry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
Isotyp
IgG2a
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... CSPG4(1464)
Spezifität
Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984). Reacts with human melanoma, glioma and proliferating brain endothelial cells.
Immunogen
Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose (Morgan et al., 1981).
Anwendung
Research Category
Zellstruktur
Zellstruktur
Research Sub Category
ECM-Proteine
ECM-Proteine
Detect Chondroitin Sulfate Proteoglycan using this Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27 validated for use in FC, IF & IP.
Immunofluorescence microscopy of cells in culture (Bumoi et al., 1984; Schrappe et al., 1992). Also, Legg J et al (2003) Development 130:6049-6063 {3% PFA fixed frozen sections}.
Immunoelectron microscopy (Bumoi et al., 1984)
Immunoprecipitation: 1μg/10E6 cells (Bumoi et al., 1984; Morgan et al., 1981)
FACS analysis: 1-2μg/10E6 cells
Functional block of melanoma cell spreading (Morgan et al., 1981), glioma cell proliferation3 and tumor growth in nude mice (Morgan et al., 1981; Schrappe et al., 1992).
Optimal working dilutions must be determined by end user.
Immunoelectron microscopy (Bumoi et al., 1984)
Immunoprecipitation: 1μg/10E6 cells (Bumoi et al., 1984; Morgan et al., 1981)
FACS analysis: 1-2μg/10E6 cells
Functional block of melanoma cell spreading (Morgan et al., 1981), glioma cell proliferation3 and tumor growth in nude mice (Morgan et al., 1981; Schrappe et al., 1992).
Optimal working dilutions must be determined by end user.
Physikalische Form
Format: Purified
Purified immunoglobulin in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Lagerung und Haltbarkeit
Maintain between 2 and 8°C in undiluted aliquots for up to 6 months.
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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