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Merck

IF014

Sigma-Aldrich

Interferon-β Protein, Recombinant human

Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a).

Synonym(e):

Fibroblast Interferon

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About This Item

UNSPSC-Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77

Biologische Quelle

human

Qualitätsniveau

Rekombinant

expressed in CHO cells

Assay

≥95% (one N-glycosylation site)

Form

liquid

Spezifische Aktivität

3.99 x 108 U/mg

Hersteller/Markenname

Chemicon®

Konzentration

1 x 106 units/mL

Methode(n)

cell culture | mammalian: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Allgemeine Beschreibung

Product Source: cDNA obtained from human fibroblast mRNA expressed in CHO cells.
Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a). Molecular weight is ~23 kDa.

Verlinkung

Replaces: IF008

Physikalische Form

Frozen liquid in 50 mM NaOAc, pH 5.5, containing 0.1% BSA.

Lagerung und Haltbarkeit

After receipt, this product should be kept at -70°C or below for retention of full activity.
Thaw product vial by incubation cold tap water until just thawed. Thawed IFNβ should be
stored in working aliquots at -70°C for up to six months. Avoid multiple freeze/thaw cycles.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the
product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Hinweis zur Analyse

Specific Activity: Determined by assaying inhibition of virus-induced cytopathic effect.In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%.The units are determined with respect to the international reference standard for human interferon beta (Hu-IFNbeta) provided by the National Institutes of Health.Unit of activity measured on VERO cells with vesicular stomatitis virus (VSV).

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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David A Constant et al.
ImmunoHorizons, 6(7), 416-429 (2022-07-06)
The three types of IFN have roles in antimicrobial immunity and inflammation that must be properly balanced to maintain tissue homeostasis. For example, IFNs are elevated in the context of inflammatory bowel disease and may synergize with inflammatory cytokines such

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