GF306

IGF-I Protein, Human Recombinant Animal Free

The Insulin-like growth factor I protein(or IGF-1 protein), also known as somatomedin C, is the dominant effector of growth hormone & is structurally homologous to proinsulin. Manufactured using all non-animal reagents.

• Synonym(e): IGF-1, IGF1, Insulinlike Growth Factor I, Insulinlike Growth Factor 1, Mechano growth factor, Somatomedin-C, IGFI
• UNSPSC Code: 12352202
• NACRES: NA.77
• eCl@ss: 32160405
• Assay: >98% (SDS-PAGE and HPLC)

Eigenschaften

• Quality Level: 100
• assay: >98% (SDS-PAGE and HPLC)
• impurities: <0.1 μg/mg protein endotoxin (<1EU/μg)
• NCBI accession no.: NM_000618.2
• UniProt accession no.: P05019
• shipped in: dry ice
• storage temp.: -10 to -25°C

Beschreibung

General description

Insulinlike growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds.

Product Source: E. coli

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Cross Reactivty
Mouse, Rat, Horse, Pig, Cow, Sheep, Monkey, Chicken, Tiger Salamander, Bacteria, Frog

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human IGF-I is manufactured using all non-animal reagents

Preparation Note

Store at -20°C for up to 6 months from date of receipt.

Analysis Note

Bioactivity assay: The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable