AB6001

Anti-MMP-9 Antibody, NT

from rabbit, purified by affinity chromatography

• Synonym(e): 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, macrophage gelatinase, matrix metallopeptidase 9, matrix metalloproteinase 9, type V collagenase
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: canine, human
• Speziesreaktivität (Voraussage durch Homologie): primate (based on 100% sequence homology), pig (based on 100% sequence homology), dog (based on 100% sequence homology), rat (based on 100% sequence homology), horse (based on 100% sequence homology), monkey (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_004985.2
• UniProt-Hinterlegungsnummer: P14780
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MMP9(4318)

Beschreibung

Allgemeine Beschreibung

All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+ binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases).

MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.

Spezifität

The antibody recognizes human MMP-9. It does not cross react with MMP-1 or MMP-2.

Immunogen

Epitope: N-terminus

KLH-conjugated synthetic peptide corresponding to the N-terminus of MMP-9.

Anwendung

Research Category
Zellstruktur

Research Sub Category
MMPs & TIMPs

Anti-MMP-9 Antibody, N-terminus is an antibody against MMP-9 for use in WB.

Qualität

Evaluated on a representative lot by Western blot using an HL-60 cell lysate supplemented with a PMA conditioned media.

Western Blot Analysis: 0.5 µg/ml of this antibody detected MMP-9 on 10 µg of HL-60 plus PMA conditioned media.

Zielbeschreibung

92kDa (pro-form) and 88kDa (active form)

Verlinkung

Replaces: AB805

Physikalische Form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
HL-60 plus PMA conditioned media

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable