615700
Total Antioxidant Status Assay Kit
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About This Item
UNSPSC-Code:
41116133
NACRES:
NA.84
Empfohlene Produkte
Verwendung
sufficient for 50 tests
Qualitätsniveau
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
assay range
standard curve range: 0-2.5 mM
(plasma)
Aufnahme
sample type serum
sample type plasma
sample type tissue extract(s)
sample type beverage(s)
sample type plant extract(s)
Nachweisverfahren
colorimetric
Versandbedingung
ambient
Lagertemp.
2-8°C
Allgemeine Beschreibung
A colorimetric assay kit for the measurement of the total antioxidant status. Each kit is suitable for performing a minimum of 50 assays.
Note: Spectrophotometer equipped with a temperature-controlled cuvette holder is strongly recommended for use with this kit.
Komponenten
Substrate, Chromogen, Standard, Buffer, and a user protocol.
Warnhinweis
Toxicity: Standard Handling (A)
Prinzip
The Calbiochem Total Antioxidant Status Assay Kit is designed to assay antioxidant levels in serum or plasma samples. Additionally, the assay may be used to measure the antioxidant potential of (suitably solubilized) food and drug samples.
Angaben zur Herstellung
• Chromogen: Add 10 ml Buffer to each vial of Chromogen needed for the assay (10 tests per vial). After reconstitution, the Chromogen is stable for 2 days at 4°C or 8 h at room temperature.
• Substrate: Dilute each vial of Substrate needed for the assay by adding 7.5 ml Buffer. After dilution, the substrate is stable for 24 h at 4°C. NOTE: Some automated methods do not require substrate dilution.
• Standard: Add 1 ml distilled water to each vial of Standard needed for the assay. After reconstitution, the Standard is stable for 2 days at 4°C or 1 month at -20°C.
• Substrate: Dilute each vial of Substrate needed for the assay by adding 7.5 ml Buffer. After dilution, the substrate is stable for 24 h at 4°C. NOTE: Some automated methods do not require substrate dilution.
• Standard: Add 1 ml distilled water to each vial of Standard needed for the assay. After reconstitution, the Standard is stable for 2 days at 4°C or 1 month at -20°C.
Lagerung und Haltbarkeit
Upon arrival store the entire kit contents at 4°C. Note: The concentration of the standard is lot-specific. Please refer to the vial label for lot-specific concentration.
Hinweis zur Analyse
Calculation of Total Antioxidant StatusThe antioxidant concentration of the Standard is used to calculate the antioxidant levels in the samples.
1. Determine the ΔA for the samples, standard, and blank. ΔA = A - Ao
2. Calculate the concentration of antioxidants in the sample using the formula below:
<div class="Bio_doc_image">Figure 2: Antioxidant Concentration Formula
</div>
NOTE: If the antioxidant concentration is greater than 2.5 mM dilute the sample with 0.9% NaCl and re-assay. Typical antioxidant levels in normal human plasma are 1.30-1.77 mM.
1. Determine the ΔA for the samples, standard, and blank. ΔA = A - Ao
2. Calculate the concentration of antioxidants in the sample using the formula below:
<div class="Bio_doc_image">Figure 2: Antioxidant Concentration Formula
</div>
NOTE: If the antioxidant concentration is greater than 2.5 mM dilute the sample with 0.9% NaCl and re-assay. Typical antioxidant levels in normal human plasma are 1.30-1.77 mM.
Sonstige Hinweise
Miller, N.J., et al. 1993. Clin. Science 84, 407.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
10 - Combustible liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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