5.09721

1α,25-Dihydroxy Vitamin D3

InSolution, ≥99%

• Synonym(e): InSolution Vitamin D3, 1α, 25-Dihydroxy-, 1,25-(OH)₂-D₃
• Empirische Formel (Hill-System): C₂₇H₄₄O₃
• Molekulargewicht: 416.64
• UNSPSC-Code: 12352205
• NACRES: NA.21

Eigenschaften

• Qualitätsniveau: 200
• Assay: ≥99% (HPLC)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Active hormonal form of vitamin D, formed by the renal 1-hydroxylation of 25-(OH)D₃. Has immunomodulatory properties in vitro and in vivo. Prevents the development of clinical diabetes in NOD mice, which are an animal model of human autoimmune diabetes. Induces reversible hydrolysis of the sphingomyelin cycle and generates ceramide. Stimulates intestinal Ca²⁺ and phosphate transport and mobilizes Ca²⁺ from bone. Involved in cellular differentiation, carcinogenesis, and insulin secretion.

Anwendung

• Evaluation system for cell-permeable CYP3A4 inhibitory activity using 1α,25-dihydroxy-vitamin D(3)-induced intestinal cell lines.: Ueno et al. developed an evaluation system for cell-permeable CYP3A4 inhibitory activity utilizing 1α,25-dihydroxy-vitamin D3-induced intestinal cell lines. This system helps in screening for CYP3A4 inhibitors, which are critical in drug metabolism and interaction studies (Ueno et al., 2021).

Biochem./physiol. Wirkung

Primary Target
vitamin D receptor

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

A 1 mM (50 ug/112 µL) solution of Vitamin D3, 1α, 25-Dihydroxy- (Cat. No. 679101) in DMSO.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Sonstige Hinweise

Semizarov, D., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15412.
Hannun, Y.A. 1994. J. Biol. Chem.269, 3125.
Mathieu, C., et al. 1994. Diabetologica37, 552.
Thomasset, M. 1994. Pathol. Biol.42, 163.
Horton, W.E., Jr., et al. 1991. J. Biol. Chem.266, 24804.
Larsen, C.G., et al. 1991. Biochem. Biophys. Res. Commun.176, 1020.
Thavarajah, M., et al. 1991. Biochem. Biophys. Res. Commun.176, 1189.
Wakasugi, M., et al. 1991. Prostaglandins42, 127.
Matsumoto, K., et al. 1990. Biochem. Biophys. Res. Commun.166, 916.
Simpson, R.U., et al. 1989. J. Biol. Chem.264, 19710.
DeLuca, H.F., and Schnoes, H.K. 1983. Annu. Rev. Biochem.52, 411.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): 188.6 °F - (refers to pure substance)
• Flammpunkt (°C): 87 °C - (refers to pure substance)