14-173-M
MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg
Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.
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About This Item
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Rekombinant
expressed in E. coli
Mol-Gew.
Mw 67.8 kDa
Hersteller/Markenname
Upstate®
Methode(n)
activity assay: suitable (kinase)
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Allgemeine Beschreibung
N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).
Product Source: recombinant, mouse, full-length MAP Kinase 2
Biochem./physiol. Wirkung
Protein Target: MAPK2
Target Sub-Family: CMGC
Verpackung
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.
Qualität
Routinely evaluated by phosphorylation myelin basic protein
Physikalische Form
Glutathione agarose; Ni2+/NTA agarose
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Lagerung und Haltbarkeit
Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.
Sonstige Hinweise
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Rechtliche Hinweise
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Skin Sens. 1
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
The Journal of Biological Chemistry, 265, 52-57 (1990)
The Journal of biological chemistry, 273(45), 29879-29887 (1998-10-29)
The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in virus replication and infectivity. Here we show that Vif is phosphorylated and regulated by p44/42 mitogen-activated protein kinase (MAPK). Vif phosphorylation by MAPK was demonstrated in
Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation.
Biochimica et Biophysica Acta, 1095, 153-160 (1991)
Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.
European Journal of Biochemistry, 212, 581-588 (1993)
The Journal of biological chemistry, 272(20), 13397-13402 (1997-05-16)
Aggregation of the high affinity IgE receptor (FcepsilonRI) in a mast cell line resulted in activation of the p42 and the stress-activated p38 mitogen-activated protein (MAP) kinases. Selective inhibition of these respective kinases with PD 098059 and SB 203580 indicated
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