861049

Azur-A-Chlorid

certified by the Biological Stain Commission, Dye content 70 %

• Synonym(e): Methylene blue
• Empirische Formel (Hill-System): C₁₄H₁₄ClN₃S
• CAS-Nummer: 531-53-3
• Molekulargewicht: 291.80
• Farbindexnummer: 52005
• Beilstein: 3922287
• EG-Nummer: 208-510-7
• MDL-Nummer: MFCD00012112
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 329769437
• NACRES: NA.47

Eigenschaften

• Qualität: certified by the Biological Stain Commission
• Qualitätsniveau: 200
• Form: powder
• Zusammensetzung: Dye content, 70%
• Farbe: blue to very dark blue; very dark green
• mp (Schmelzpunkt): 290 °C (dec.) (lit.)
• Löslichkeit: H₂O: 1 mg/mL
• λmax: 633 nm
• ε (Extinktionskoeffizient): ≥11000 at 242-246 nm in H₂O at -1; ≥26000 at 287-291 nm in H₂O; ≥34000 at 620-634 μm in H₂O
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• InChIKey: NALREUIWICQLPS-UHFFFAOYSA-N

Beschreibung

Anwendung

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.

Biochem./physiol. Wirkung

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Eignung

Zertifiziert für den Einsatz in Lillies modifizierter Nocht-Färbung und der NCCLS-Methode für Blutausstriche.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)