Key Documents

View All Documentation

Pyrophosphatase, Inorganic from Escherichia coli

Name: Pyrophosphatase, Inorganic from <I>Escherichia coli</I>
Brand: SIGMA

recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein

Synonym(s):

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

CAS Number:

9024-82-2

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

232-784-7

MDL number:

MFCD00131379

EC Number:

3.6.1.1 (BRENDA, IUBMB)

Specific activity:

≥800 units/mg protein

Assay:

≥90%

Recombinant:

expressed in E. coli

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

recombinant

expressed in E. coli

Quality Segment

200

assay

≥90%

form

lyophilized powder

specific activity

≥800 units/mg protein

mol wt

hexamer subunit mol wt 20 kDa

storage temp.

−20°C

Description

General description

Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.

Application

Pyrophosphatase, Inorganic from Escherichia coli has been used as a component of transcription buffer.

Pyrophosphatase, inorganic from Escherichia coli has been used in assay for conjugation of ubiquitin and ubiquitin-like proteins. It has been used for one-pot three-enzyme system for synthesis of Lewis x and sialyl Lewis x antigens.

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H₂O → 2P_i.
It plays an important role in protein, RNA, and DNA synthesis.

Biochem/physiol Actions

Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium.

Physical form

Lyophilized powder in Tris-buffered salts containing protease inhibitors

Other Notes

A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg²⁺ dependent. It is a relatively thermostable protein.

One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	SAE0215	I1643	P4069
Sigma-Aldrich I5907 Pyrophosphatase, Inorganic from Escherichia coli	Sigma-Aldrich SAE0215 Inorganic Pyrophosphatase from Escherichia coli	Sigma-Aldrich I1643 Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)	Sigma-Aldrich P4069 Phosphatase, Alkaline from Escherichia coli
description recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein	description ≥100 units/mL, buffered aqueous solution	description powder, ≥500 units/mg protein (E1%/280)	description buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
specific activity ≥800 units/mg protein	specific activity ≥100 units/mL	specific activity ≥500 units/mg protein (E1%/280)	specific activity 20-50 units/mg protein (in glycine buffer)
assay ≥90%	assay ≥95% (size exclusion chromatography)	assay -	assay -
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant -	recombinant -
form lyophilized powder	form buffered aqueous solution	form powder	form buffered aqueous glycerol solution
mol wt hexamer subunit mol wt 20 kDa	mol wt 19.7 kDa	mol wt 71 kDa	mol wt -
storage temp. −20°C	storage temp. -10 to -25°C	storage temp. −20°C	storage temp. −20°C