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Phosphatase, Alkaline from Escherichia coli

Name: Phosphatase, Alkaline from <I>Escherichia coli</I>
Brand: SIGMA

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

Synonym(s):

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:

9001-78-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-631-4

MDL number:

MFCD00131849

EC Number:

3.1.3.1 (BRENDA, IUBMB)

eCl@ss:

42010105

Specific activity:

20-50 units/mg protein (in glycine buffer)

Biological source:

Escherichia coli

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Properties

biological source

Escherichia coli

Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

20-50 units/mg protein (in glycine buffer)

technique(s)

protein detection: suitable

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Description

Application

Phosphatase, Alkaline from Escherichia coli has been used:

in fluorometric assay to determine its enzyme activity
in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.

Biochem/physiol Actions

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Solution in 5 mM Tris-HCl, pH 7.4, 0.5 mM MgCl₂, 0.5 mM ZnCl₂, 50% (w/v) glycerol

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Disclaimer

Alkaline phosphatase in 5 mM EDTA, pH 8, will be irreversibly heat inactivated at 75°C for 10 minutes.

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This Item	P5931	P4252	A2237
Sigma-Aldrich P4069 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P5931 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P4252 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich A2237 Phosphatase, Alkaline shrimp
description buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)	description lyophilized powder, 30-60 units/mg protein (in glycine buffer)	description ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)	description ≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host
specific activity 20-50 units/mg protein (in glycine buffer)	specific activity 30-60 units/mg protein (in glycine buffer)	specific activity 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)	specific activity -
biological source Escherichia coli	biological source -	biological source Escherichia coli	biological source -
technique(s) protein detection: suitable	technique(s) protein detection: suitable	technique(s) protein detection: suitable	technique(s) protein detection: suitable
form buffered aqueous glycerol solution	form lyophilized powder	form ammonium sulfate suspension	form buffered aqueous glycerol solution
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
foreign activity DNase, RNase, none detected	foreign activity -	foreign activity -	foreign activity RNAse, DNAse, none detected

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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