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CRISPR-Lenti Human HPRT1 Positive Control DNA

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About This Item

NACRES:

NE.02

UNSPSC Code:

41106609

Pricing and availability is not currently available.

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Properties

packaging

vial of 50 μL

Quality Segment

200

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR
CRISPR

shipped in

dry ice

storage temp.

−20°C

Description

Application

Functional Genomics/Screening/Target Validation

Positive control, HPRT1, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit Sigma.com/crispr.

Features and Benefits

Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPR DNA. The number of cells with HPRT1 knocked out increases over time with 6−TG selection.

Physical form

The Sigma CRISPR Lenti Human HPRT1 Positive Control Vector is provided as 1 μg of plasmid DNA in Tris−EDTA (TE).

Legal Information

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

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Show Differences

1 of 1

This Item	CRISPR13V	CRISPR21	CRISPR13H
Sigma-Aldrich CRISPR13 CRISPR-Lenti Human HPRT1 Positive Control DNA	Sigma-Aldrich CRISPR13V CRISPR-Lenti Human HPRT1 Positive Control Transduction Particles	Sigma-Aldrich CRISPR21 Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector)	Sigma-Aldrich CRISPR13H CRISPR-Lenti Human HPRT1 Positive Control High Titer Transduction Particles
description -	description -	description -	description -
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −20°C	storage temp. −70°C	storage temp. −20°C	storage temp. −70°C
packaging vial of 50 μL	packaging vial of 200 μL	packaging vial of 50 μL	packaging vial of 200 μL
application(s) CRISPR	application(s) CRISPR	application(s) CRISPR	application(s) CRISPR
concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)	concentration 1x10⁶ VP/ml (via p24 assay)	concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)	concentration 5x10⁸ VP/ml (via p24 assay)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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