A4486

AMPK (α1/β1/γ3), active, His tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): HAMPKb, MGC17785, MGC33776, MGC57364, PRKAA1, PRKAB1, PRKAG3
• NACRES: NA.77
• UNSPSC Code: 12352200
• Specific activity: 157-213 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 157-213 nmol/min·mg
• mol wt: A1 subunit ~68 kDa, B1 subunit ~38 kDa, G3 subunit ~51 kDa
• UniProt accession no.: Q13131, Q9UGI9, Q9Y478
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PRKAA1(5562), PRKAB1(5564), PRKAG3(53632)

Description

Biochem/physiol Actions

AMPK (A1/B1/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and 3-hydroxy-3-methylglutaryl-CoA reductase. The γ subunit is dominantly expressed in skeletal muscle where it may play a key role in the regulation of energy metabolism.

Physical form

Supplied in 50 mM Sodium Phosphate, pH 7.0, with 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H360D
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable