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Acylase I from porcine kidney

Name: Acylase I from porcine kidney
Brand: SIGMA

Grade I, lyophilized powder, ≥1500 units/mg protein

Synonym(s):

Aminoacylase, N-Acylamino acid amidohydrolase

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About This Item

CAS Number:

9012-37-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-732-3

MDL number:

MFCD00081285

EC Number:

3.5.1.14 (BRENDA, IUBMB)

Specific activity:

≥1500 units/mg protein

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Properties

type

Grade I

Quality Segment

200

form

lyophilized powder

specific activity

≥1500 units/mg protein

composition

Protein, ≥60%

UniProt accession no.

P37111

storage temp.

−20°C

Gene Information

pig ... ACY1(396930)

Description

Application

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .

Biochem/physiol Actions

Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

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This Item	A8376	P6675	K7250
Sigma-Aldrich A3010 Acylase I from porcine kidney	Sigma-Aldrich A8376 Acylase I from porcine kidney	Sigma-Aldrich P6675 Prolidase from porcine kidney	Sigma-Aldrich K7250 Kidney acetone powder porcine
description Grade I, lyophilized powder, ≥1500 units/mg protein	description Grade II, salt-free, lyophilized powder, 300-1,500 units/mg protein	description lyophilized powder, ≥100 units/mg protein	description Type II
Gene Information pig ... ACY1(396930)	Gene Information pig ... ACY1(396930)	Gene Information -	Gene Information -
specific activity ≥1500 units/mg protein	specific activity 300-1,500 units/mg protein	specific activity ≥100 units/mg protein	specific activity -
form lyophilized powder	form salt-free, lyophilized powder	form lyophilized powder	form -
UniProt accession no. P37111	UniProt accession no. P37111	UniProt accession no. -	UniProt accession no. -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Codes

H315,H319,H334,H335

Precautionary Statements

P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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