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Acylase I from porcine kidney

Name: Acylase I from porcine kidney
Brand: SIGMA

Grade II, salt-free, lyophilized powder, 300-1,500 units/mg protein

Synonym(s):

Aminoacylase, N-Acylamino acid amidohydrolase

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About This Item

CAS Number:

9012-37-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-732-3

MDL number:

MFCD00081285

EC Number:

3.5.1.14 (BRENDA, IUBMB)

Specific activity:

300-1,500 units/mg protein

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Properties

type

Grade II

Quality Segment

200

form

salt-free, lyophilized powder

specific activity

300-1,500 units/mg protein

UniProt accession no.

P37111

storage temp.

−20°C

Gene Information

pig ... ACY1(396930)

Description

Application

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues [1]. Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids [2].

Biochem/physiol Actions

Acylase I is a zinc metalloprotein that catalyzes the kinetic resolution of unnatural and rarely occurring α-amino acids. Its enantioselectivity for the hydrolysis of N-acyl L-α-amino acids is nearly absolute, yet it accepts substrates having a wide range of structure and functionality. Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates.[1]

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

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1 of 1

This Item	A3010	C3755	C3172
Sigma-Aldrich A8376 Acylase I from porcine kidney	Sigma-Aldrich A3010 Acylase I from porcine kidney	Sigma-Aldrich C3755 Creatine Phosphokinase from rabbit muscle	Sigma-Aldrich C3172 Creatininase from microorganisms
Gene Information pig ... ACY1(396930)	Gene Information pig ... ACY1(396930)	Gene Information -	Gene Information -
specific activity 300-1,500 units/mg protein	specific activity ≥1500 units/mg protein	specific activity ≥150 units/mg protein	specific activity 100-300 units/mg protein
form salt-free, lyophilized powder	form lyophilized powder	form salt-free, lyophilized powder	form lyophilized powder
UniProt accession no. P37111	UniProt accession no. P37111	UniProt accession no. -	UniProt accession no. -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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