GC Analysis of Volatiles in Limoncello on SLB®-5ms after SPME using a 100 μm PDMS Fiber
Materials
SPME 파이버
SPME fiber assembly Polydimethylsiloxane (PDMS)
df 100 μm(PDMS, needle size 24 ga, for use with manual holder관련 제품
분석 컬럼
표준 물질
CONDITIONS
sample/matrix
Limoncello diluted 1:100 in water, then 5 mL of this solution transferred to a 10 mL crimped vial spiked with 100 μL of a 100 ppm solution of nonane (internal standard)
SPME fiber
100 μm polydimethylsiloxane (57300-U)
extraction
headspace, with a pre-equilibration period of 10 min. at 40 °C, and then a fiber exposure of 30 min. at 40 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 ppm
desorption process
250 °C for 5 min
column
SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven
40 °C, 3 °C/min to 250 °C (2 min)
inj. temp.
250 °C
detector
FID, 260 °C (sampling rate 80 ms)
carrier gas
helium, 35.0 cm/sec
injection
splitless
liner
0.75 mm I.D. SPME (2633901)
sample
Limoncello liquor
설명
분석 메모
In this application, SPME was used for the analysis of volatiles in limoncello using a headspace method. SPME is ideal for the analysis of aroma volatiles in flavored beverages such as liquors. A 100 μm PDMS fiber was inserted into the headspace of the limoncello sample for 30 minutes at 40 °C followed by GC analysis using FID detection. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.
법적 정보
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany