기술
HPLC: suitable
검사 파라미터
column: Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (A777028)
mobile phase: [A], 0.15 M potassium phosphate, monobasic, pH 7.0 with potassium hydroxide; [B] 0.15 M potassium phosphate, monobasic, 0.2 M arginine, pH 7.0 with phosphoric acid (mobile phase is either 100% A or 100% B)
flow rate: 0.3 mL/min
pressure: 1030 psi 100% A (71 bar)
pressure: 1165 psi 100% B (80 bar)
column temp.: 25 °C
detector: UV, 280 nm
injection: 2.5 μL
sample: Waters BEH200 SEC protein standard
적합성
application for HPLC
응용 분야
life science and biopharma
분석 메모
Arginine can have benefitial effects on peak shape and recovery of hydrophobic proteins when chromatographed by size exclusion chromatography. In this case, with protein standards that are not particularly hydrophobic, no dramatic affect is noted, as assessed by peak efficiency, peak widths, or peak retention.
법적 정보
Zenix is a registered trademark of Sepax Technologies
분석물
설명
Thyroglobulin from bovine thyroid, (For Gel Filtration Chromatography)
IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Bovine Serum Albumin, lyophilized powder, ≥96% (agarose gel electrophoresis)
Myoglobin from equine heart, ≥90% (SDS-PAGE), essentially salt-free, lyophilized powder
Uracil, ≥99.0%
부속물
분석 컬럼
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