추천 제품
설명
Phenyl-650M
제품 라인
TOYOPEARL®
형태
slurry
포장
bottle of 100 mL
파라미터
≤50% organic solvent
3 bar max. pressure
기술
HPLC: suitable
기질
polymer (65 μm)
기질 활성군
phenyl phase
입자 크기
65 μm
작동 pH
1-13
분리 기술
hydrophobic interaction (HIC)
일반 설명
Toyopearl Phenyl-650 resin is the middle hydrophobicity of the five HIC ligands offered by Tosoh Bioscience. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSKgel Phenyl-5PW resin is suggested. Both products have the same selectivity.
애플리케이션
Toyopearl media are used in separation media and resins. Toyopearl media offer high yield recovery of proteins, using various aqueous eluants.
규격
Clean in place with 0.5 M NaOH or 0.1 M HCl.
물리적 형태
Shipped in 20% (v/v) ethanol.
법적 정보
Toyopearl is a registered trademark of Tosoh Corporation
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Flam. Liq. 3
WGK
WGK 1
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
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Tara Tibbs Jones et al.
Biotechnology and bioengineering, 87(3), 388-399 (2004-07-29)
Interesting retention and selectivity changes have been noted for a number of proteins in hydrophobic interaction chromatography (HIC). In this study, we investigated the degree to which conformational changes may be responsible for selectivity changes of stable proteins. Hydrogen-deuterium isotope
P Ceccaroli et al.
Journal of chromatography. B, Biomedical sciences and applications, 702(1-2), 41-48 (1998-02-04)
Hydrophobic interaction chromatography (HIC) has been used extensively for the separation of proteins and peptides by elution using a descending salt gradient, with and without the use of detergents or denaturing agents. In this paper we compare different hydrophobic interaction
V Stocchi et al.
Journal of chromatography. A, 676(1), 51-63 (1994-07-29)
Hydrophobic interaction chromatography (HIC) has been employed extensively in the separation of proteins by elution using a descending salt gradient, with and without the use of detergents or denaturing agents. In this study, a new hydrophobic interaction chromatographic support, Toyopearl
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