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Merck
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문서

567501-U

Supelco

Discovery® HS F5 (3 µm) HPLC Columns

L × I.D. 3.3 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC 코드:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 3.3 cm × 2.1 mm

material

stainless steel column

Agency

suitable for USP L43

제품 라인

Discovery®

특징

endcapped

제조업체/상표

Discovery®

포장

1 ea of

라벨링 범위

12% Carbon loading

파라미터

≤70 °C temp. range
400 bar pressure (5801 psi)

기술

HPLC: suitable
LC/MS: suitable

길이 × I.D.

3.3 cm × 2.1 mm

표면적

300 m2/g

표면 범위

4 μmol/m2

불순물

<10 ppm metals

기질

silica gel, high purity, spherical particle platform
fully porous particle

기질 활성군

PFP (pentafluorophenyl) phase

입자 크기

3 μm

공극 크기

120 Å

operating pH range

2-8

응용 분야

food and beverages

분리 기술

reversed phase

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관련 카테고리

일반 설명

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

특징 및 장점

  • Unique selectivity
  • Similar retention to C18 (sometimes requires stronger mobile phase)
  • Excellent peak shape
  • Stable, low-bleed LC-MS separations
  • Scalable separations from 3 to 10μm particle sizes

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

시험 성적서(COA)

Lot/Batch Number
287906-06
287906-05
287906-04
287906-03
287906-02

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Jana Maláková et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)
Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation
Jennie Wang et al.
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An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
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관련 콘텐츠

Discovery® HPLC Columns for Fast LC-MS Analysis

Discovery® HPLC Columns for Fast LC-MS Analysis. The Discovery LC-MS line offers short length columns for fast LC-MS analysis that are rugged and dependable. Supelco offers a robust product that withstands column shock due to fast gradient turnaround times.

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