Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis Express RP-Amide is 3.0 micromoles/meter-squared.
₩761,047
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추천 제품
제품명
Ascentis® Express RP-Amide, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 3 cm × 4.6 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L60
제품 라인
Ascentis®
특징
endcapped
제조업체/상표
Ascentis®
포장
1 ea of
파라미터
60 °C temp. range
600 bar max. pressure (9000 psi)
기술
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
길이 × I.D.
3 cm × 4.6 mm
표면적
135 m2/g
불순물
<5 ppm metals
Matrix
Fused-Core® particle platform
superficially porous particle
기질 활성군
amide, alkyl phase
입자 크기
2.7 μm
공극 크기
90 Å
작동 pH 범위
2-9
응용 분야
food and beverages
분리 기술
reversed phase
유사한 제품을 찾으십니까? 방문 제품 비교 안내
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
가아드 카트리지
관련 제품
애플리케이션
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
Hirotaka Kushida et al.
Biomedical chromatography : BMC, 27(12), 1647-1656 (2013-07-03)
Uncaria Hook (UH) alkaloids are involved in the beneficial effects of Yokukansan. However, the pharmacokinetics of UH alkaloids after oral administration of Yokukansan has not yet been sufficiently investigated. Therefore, we developed and validated a sensitive and specific high-performance liquid
S Fan et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 30(12), 2093-2101 (2013-10-22)
A liquid chromatography-linear ion-trap spectrometry (LC-MS³) method using β-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 β₂-agonists and 21 β-blockers in urine samples. Urine samples were subjected to enzymatic
Fan Sai et al.
Journal of agricultural and food chemistry, 60(8), 1898-1905 (2012-02-15)
A sensitive method has been developed for the simultaneous determination of residues of 25 β₂-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry (HPLC-LIT-MS). This method is based on a new
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
문서
Innovative particle technology and RP-Amide bonding chemistry enhance chromatography performance for improved peak shape and resolution.
A large variety of cannabinoid drug standards, along with an HPLC method with the Supelco Ascentis® Express RP-Amide column, are presented in this article.
Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.
Chromatograms
application for HPLCapplication for HPLC-
What is the carbon load for Ascentis Express RP-Amide?
1 답변-
도움이 되었습니까?
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 답변-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
도움이 되었습니까?
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Can I use Ascentis Express on a UHPLC system?
1 답변-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
도움이 되었습니까?
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 답변-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
도움이 되었습니까?
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 답변-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)
도움이 되었습니까?
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Can I use Ascentis Express on any type of HPLC system?
1 답변-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 답변-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
도움이 되었습니까?
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 답변-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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